Fresh Cells from Mouse Spinal Cord in Suspension, as Tissue and on MEA Plates
Quality Check
Cultures in suspension
Cultures in suspension have high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates
Pre-cultured microelectrode array plates are tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling
Primary neuronal cell cultures from the spinal cord are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance. Spinal cord cultures are used in pain research and for models of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS).
Spinal Cord.
Spinal Cord cultures are dissected from the spinal cord with or without dorsal root ganglia. Preparation day is at embryonic day 18, E18. The cultures consist of neurons and glial cells, mainly astrocytes and microglia 28 days after plating. These spinal cord preparations contain GABAergic, glutamatergic cells, and motor neurons. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors. They are capsaicin-sensitive.
Applications
Toxicity and Safety Pharmacology
Spinal cord cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more.
Pharmacology
Primary spinal cord cultures are sensitive to broad classes of drugs. They can be used to test compounds for their analgesic effects. They contain receptors that are relevant for pain and pruritus.
Quality Check
Cultures in suspension
Cultures in suspension have high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates
Pre-cultured microelectrode array plates are tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling
Primary neuronal cell cultures from the spinal cord are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance. Spinal cord cultures are used in pain research and for models of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS).
Spinal Cord.
Spinal Cord cultures are dissected from the spinal cord with or without dorsal root ganglia. Preparation day is at embryonic day 18, E18. The cultures consist of neurons and glial cells, mainly astrocytes and microglia 28 days after plating. These spinal cord preparations contain GABAergic, glutamatergic cells, and motor neurons. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors. They are capsaicin-sensitive.
Applications
Toxicity and Safety Pharmacology
Spinal cord cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more.
Pharmacology
Primary spinal cord cultures are sensitive to broad classes of drugs. They can be used to test compounds for their analgesic effects. They contain receptors that are relevant for pain and pruritus.
Spinal Cord 191101