Fresh Cells from Frontal Cortex in Suspension, as Tissue and on MEA Plates
Frontal cortex.
Frontal cortex cultures have been dissected from embryonic cortices. Preparation day is at embryonic day 16, E16. These cultures consist of approximately 20% neurons and 80% glial cells, mainly astrocytes and also 1% microglia 28 days after plating. These frontal cortex preparations contain GABAergic and glutamatergic cells. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors.
Quality Check
Cultures in suspension
Cultures in suspension have high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates
Pre-cultured microelectrode array plates will be tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling
Primary neuronal cell cultures from the frontal cortex are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance.
Primary neuronal cell cultures from the frontal cortex are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance.
Applications
Toxicity and Safety Pharmacology
Primary frontal cortex cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more.
Frontal cortex cultures were also used for assays to predict a compound’s safety profile of seizurogenecity and sedation, read more. Seizurogenecity is an unwanted side effect of drugs and a cause of failed clinical trials. Compounds can provoke ictal events as seizures in frontal cultures, detectable with microelectrode arrays or C2+-imaging. Frontal cortex cultures are sensitive to most hazardous compounds acting via the central nervous system.
Neurodevelopment
Primary neuronal cell cultures are precisely reflecting neurodevelopmental processes. Primary neural cell cultures, prepared at embryonic day 16, represent physiological adequate model systems. They follow developmental processes in-vivo, as shown in a wide range of studies. For example, the switch between the GABA-A subtypesalpha1, alpha 3, and alpha5 is in concordance with the in vivo situation, to name only one aspect.
… read more
Pharmacology
Primary frontal cortex cultures are sensitive to broad classes of drugs. They act via ionotropic or metabotropic receptors or other drug targets. The cultures comprise a wide variety of receptors and are essential tools to study poly-pharmacology. ... read more
Primary frontal cortex cultures are sensitive to broad classes of drugs. They act via ionotropic or metabotropic receptors or other drug targets. The cultures comprise a wide variety of receptors and are essential tools to study poly-pharmacology. ... read more
Frontal cortex.
Frontal cortex cultures have been dissected from embryonic cortices. Preparation day is at embryonic day 16, E16. These cultures consist of approximately 20% neurons and 80% glial cells, mainly astrocytes and also 1% microglia 28 days after plating. These frontal cortex preparations contain GABAergic and glutamatergic cells. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors.
Quality Check
Cultures in suspension
Cultures in suspension have high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates
Pre-cultured microelectrode array plates will be tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling
Primary neuronal cell cultures from the frontal cortex are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance.
Primary neuronal cell cultures from the frontal cortex are usable for in-vitro disease models. These disease models are very predictive because of their physiological relevance.
Applications
Toxicity and Safety Pharmacology
Primary frontal cortex cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more.
Frontal cortex cultures were also used for assays to predict a compound’s safety profile of seizurogenecity and sedation, read more. Seizurogenecity is an unwanted side effect of drugs and a cause of failed clinical trials. Compounds can provoke ictal events as seizures in frontal cultures, detectable with microelectrode arrays or C2+-imaging. Frontal cortex cultures are sensitive to most hazardous compounds acting via the central nervous system.
Neurodevelopment
Primary neuronal cell cultures are precisely reflecting neurodevelopmental processes. Primary neural cell cultures, prepared at embryonic day 16, represent physiological adequate model systems. They follow developmental processes in-vivo, as shown in a wide range of studies. For example, the switch between the GABA-A subtypesalpha1, alpha 3, and alpha5 is in concordance with the in vivo situation, to name only one aspect.
… read more
Primary neuronal cell cultures are precisely reflecting neurodevelopmental processes. Primary neural cell cultures, prepared at embryonic day 16, represent physiological adequate model systems. They follow developmental processes in-vivo, as shown in a wide range of studies. For example, the switch between the GABA-A subtypesalpha1, alpha 3, and alpha5 is in concordance with the in vivo situation, to name only one aspect.
… read more
Pharmacology
Primary frontal cortex cultures are sensitive to broad classes of drugs. They act via ionotropic or metabotropic receptors or other drug targets. The cultures comprise a wide variety of receptors and are essential tools to study poly-pharmacology. ... read more
Primary frontal cortex cultures are sensitive to broad classes of drugs. They act via ionotropic or metabotropic receptors or other drug targets. The cultures comprise a wide variety of receptors and are essential tools to study poly-pharmacology. ... read more
Frontal Cortex - 191101
You arrive to this page because you are interested in research and drug discovery using fresh cortical neurons. Our topic areas are MEA plates and microelectrode arrays, cerebral cortical neurons and cortex culture. Our primary neurons from cortex cells are available in suspension and as neuronal cortex tissue. We are experts in MEA microelectgrode arrays, using MEA plates from Axion Biosystems.
Contact us by E-mail.
Contact us by E-mail.