Fresh Dopaminergic Cells from Midbrain in Suspension and as Tissue
Midbrain with Dopaminergic Neurons
Midbrain cultures have been dissected from the ventral part of embryonic midbrain, which contains the substantia nigra. Preparation day is at embryonic day 14, E14. The cultures contain dopaminergic neurons at high amount, glutamatergic and gabaergic neurons as well as glial cells, mainly astrocytes and also microglia after 28 days post-plating. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors, as well as dopaminergic.
Quality Check
Cultures in suspension.
Cultures in suspension show high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates Pre-cultured microelectrode array plates will be tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling with ventral Midbrain Neurons from Substantia Nigra.
Primary neuronal cell cultures from the ventral Midbrain are used for in-vitro disease models. These disease models are very predictive because of their physiological relevance. The cultures comprise a wide variety of receptors and are essential tools to study, for example in case of neurodegenerative diseases such as Parkinson’s disease. Dopaminergic neurons of substantia nigra pars compacta are highly vulnerable in Parkinson’s disease.
Please use the following links for NeuroProof's Parkinson's Disease In Vitro Disease Models and PD Screening Assays and NeuroProof's Cell Viability and Cytotoxicity Assay Services.
Applications
Toxicity and Safety Pharmacology
Midbrain cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more. Midbrain cultures are sensitive to hazardous compounds acting via the central nervous system on cholinergic and dopaminergic receptors, for example.
Pharmacology
Midbrain cultures are sensitive to broad classes of drugs as dopaminergic agonists and antagonists. Midbrain cultures contain cholinergic receptors and they are sensitive to muscarinic and nictonic receptors.
Midbrain with Dopaminergic Neurons
Midbrain cultures have been dissected from the ventral part of embryonic midbrain, which contains the substantia nigra. Preparation day is at embryonic day 14, E14. The cultures contain dopaminergic neurons at high amount, glutamatergic and gabaergic neurons as well as glial cells, mainly astrocytes and also microglia after 28 days post-plating. Pharmacological studies show that these cells are sensitive to compounds targeting ionotropic and metabotropic receptors, as well as dopaminergic.
Quality Check
Cultures in suspension.
Cultures in suspension show high viability. Quotation of the number of cells refers to viable cells. Cells are checked to be free of Mycoplasma and Klebsiella.
Tissues
Tissues are viable and have more than 70% of viable cells. Each preparation is checked to be free of Mycoplasma and Klebsiella.
Pre-cultured MEA plates Pre-cultured microelectrode array plates will be tested for activity before shipping. Only plates with 80% active wells with each more than five active units will be shipped.
Disease modeling with ventral Midbrain Neurons from Substantia Nigra.
Primary neuronal cell cultures from the ventral Midbrain are used for in-vitro disease models. These disease models are very predictive because of their physiological relevance. The cultures comprise a wide variety of receptors and are essential tools to study, for example in case of neurodegenerative diseases such as Parkinson’s disease. Dopaminergic neurons of substantia nigra pars compacta are highly vulnerable in Parkinson’s disease.
Please use the following links for NeuroProof's Parkinson's Disease In Vitro Disease Models and PD Screening Assays and NeuroProof's Cell Viability and Cytotoxicity Assay Services.
Applications
Toxicity and Safety Pharmacology
Midbrain cultures are useful tools for neurodevelopmental toxicity assays because they can be maintained in culture for a long time up to months.
They are valuable in viability and cytotoxic assays, read more. Midbrain cultures are sensitive to hazardous compounds acting via the central nervous system on cholinergic and dopaminergic receptors, for example.
Pharmacology
Midbrain cultures are sensitive to broad classes of drugs as dopaminergic agonists and antagonists. Midbrain cultures contain cholinergic receptors and they are sensitive to muscarinic and nictonic receptors.
Midbrain 20200130
Notes: This page covers specific topics related to midbrain dopaminergic neurons, midbrains technologies in general, and structures of the midbrain. With regard to substantia nigra, substantia negra in the context of substantia nigra parkinson together with the terms substantia nigra pars compacta, substantia nigra parkinson, parkinson's disease substantia nigra and substantia nigra midbrain might be relevant as well. We also cover the dopaminergic award system in conjunction to dopaminerge, dopaminerges system and midbrain dopaminergic neurons / dopaminergic drugs. We consider also glutamatergic neurons and glutamatergic system as important for our drug discovery activitites.